Registration Instructions
There are two steps to complete the registration process:
First, complete the application below. All sections must be completed. For team projects, enter the name of the student who will be the main presenter during the competition. If all students are equally presenting, then enter the name of the student who completed the most work in the project.
Second, email a one-page abstract of the science project to lemons@vt.edu. Use an Adobe PDF or Microsoft Word file only when submitting the abstract. The top of the abstract should contain the title of the science fair project and the names of the presenters in alphabetical order. Next to each person's name, include the grade level in parenthesis. Bold only the title of the project (do not bold the names). Then, include the abstract. Keep the entire document single-spaced and left justified.
Registration closes at 5:00pm on April 18, 2014. All documentation must be completed by then to qualify for this competition. Also note that there are only eight positions available for the competition, and they are given on a first-come-first-serve basis. Register as soon as possible to get your spot.
Below is an example:
Deletion of the Nuclear Gene YME1 Stabilizes Mutant Forms of Cox2p
John Doe (11), Sarah Evans (11), & Travis Forbes (12)
Cytochrome c oxidase (CcO) is the terminal protein complex in the electron transport chain, and CcO transfers electrons from the cytochrome c to oxygen to form water. Subunit II of the complex is the component that interacts directly with cytochrome c to accept the electrons. It is encoded by COX2, and missense mutations can lead to loss of respiration in yeast. In several missense COX2 mutants, the protein (Cox2p) has been shown to be rapidly degraded within the cell. The nuclear gene YME1 has been shown to degrade unassembled, wild type Cox2p. We wished to determine if deletion of YME1 could stabilize the mutant forms of Cox2p, and if stabilization could lead to recovery of respiratory capability. We have demonstrated by Western blot analysis that the disruption of YME1 stabilizes Cox2p in three different missense mutants. Further, this stabilization restored respiration to two of the three mutant strains as determined by growth on non-fermentable carbon sources. Surprisingly, one of the mutant strains which is restored to respiratory function bears an alteration in an amino acid conserved in the COX2 gene from all species examined.
First, complete the application below. All sections must be completed. For team projects, enter the name of the student who will be the main presenter during the competition. If all students are equally presenting, then enter the name of the student who completed the most work in the project.
Second, email a one-page abstract of the science project to lemons@vt.edu. Use an Adobe PDF or Microsoft Word file only when submitting the abstract. The top of the abstract should contain the title of the science fair project and the names of the presenters in alphabetical order. Next to each person's name, include the grade level in parenthesis. Bold only the title of the project (do not bold the names). Then, include the abstract. Keep the entire document single-spaced and left justified.
Registration closes at 5:00pm on April 18, 2014. All documentation must be completed by then to qualify for this competition. Also note that there are only eight positions available for the competition, and they are given on a first-come-first-serve basis. Register as soon as possible to get your spot.
Below is an example:
Deletion of the Nuclear Gene YME1 Stabilizes Mutant Forms of Cox2p
John Doe (11), Sarah Evans (11), & Travis Forbes (12)
Cytochrome c oxidase (CcO) is the terminal protein complex in the electron transport chain, and CcO transfers electrons from the cytochrome c to oxygen to form water. Subunit II of the complex is the component that interacts directly with cytochrome c to accept the electrons. It is encoded by COX2, and missense mutations can lead to loss of respiration in yeast. In several missense COX2 mutants, the protein (Cox2p) has been shown to be rapidly degraded within the cell. The nuclear gene YME1 has been shown to degrade unassembled, wild type Cox2p. We wished to determine if deletion of YME1 could stabilize the mutant forms of Cox2p, and if stabilization could lead to recovery of respiratory capability. We have demonstrated by Western blot analysis that the disruption of YME1 stabilizes Cox2p in three different missense mutants. Further, this stabilization restored respiration to two of the three mutant strains as determined by growth on non-fermentable carbon sources. Surprisingly, one of the mutant strains which is restored to respiratory function bears an alteration in an amino acid conserved in the COX2 gene from all species examined.
